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The antibody is from purified rabbit serum by affinity purification by using sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
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Overexpression of the gene has long been noticed in lots of tumors, which results in chromosome instability, and thus may perhaps lead to tumorigenesis. This protein was uncovered to associate with, and be linked to, the phosphorylation of NPAT protein (nuclear protein mapped to your ATM locus), which participates in cell-cycle controlled histone gene expression and performs a vital role in endorsing cell-cycle progression during the absence of pRB. Two alternatively spliced transcript variants of this gene, which encode distinctive isoforms, are described. Two supplemental splice variants have been reported but in-depth nucleotide sequence data is not really still accessible.
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Blocking peptides are peptides that bind exclusively into the target antibody and block antibody binding. These peptide commonly consists of the epitope identified via the antibody.
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This guide outlines even more achievable optimization of cellular click here and immunoassay parameters to be certain the very best final results are...
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This manual outlines more achievable optimization of cellular and immunoassay parameters to be sure the absolute best benefits are...
Antibodies certain to the blocking peptide no more bind on the epitope about the focus on protein. By comparing the staining from the blocked antibody compared to the antibody by yourself, you can see which staining is unique.
Concentrate on Information and facts Cyclin E belongs into the remarkably conserved cyclin relatives, whose customers are characterized by a dramatic periodicity in protein abundance in the mobile cycle. Cyclins operate as regulators of CDK kinases. Distinct cyclins exhibit distinctive expression and degradation designs which contribute to the temporal coordination of each and every mitotic function. Cyclin E kinds a fancy with and capabilities being a regulatory subunit of CDK2, whose action is necessary for cell cycle G1/S changeover. This protein accumulates in the G1-S period boundary and is also degraded as cells progress through S period.
The measurement of protein phosphorylation is a great tool for measuring the modulation of receptor activation by each antibodies...
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